About this document
Elecsys Vitamin D Total III Assay Guide by Vegha Nedya is a document available to read on EtoBox.
The document provides information on the Elecsys Vitamin D total III assay. 1) The assay uses a ruthenium-labeled vitamin D binding protein to bind 25-hydroxyvitamin D3 and D2 in a sample. A monoclonal antibody blocks cross-reactivity to 24,25-dihydroxyvitamin D. 2) The sample is pretreated to release 25-hydroxyvitamin D from vitamin D binding protein. It is then incubated with the ruthenium-labeled binding protein. 3) Unbound material is removed and chemiluminescence from the bound fraction is measur
- Author
- Vegha Nedya
- Language
- EN