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Career situation of first and presenting author Student for a master or a PhD. Introduction Systemic Sclerosis (SSc) is a rare, heterogeneous autoimmune disease characterized by microvascular damage, organ fibrosis and immune dysfunction. Autoantibodies are detected in >95% of patients, the most prevalent being anticentromere (ACA) and anti-topoisomerase (ATA) antibodies. Although used for diagnosis, little is known about the underlying auto-reactive B cell responses. In particular, the ACA B cell response has been poorly studied. Objectives Characterization of the ACA B cell response in SSc patients. Methods ACA IgG, IgA and IgM levels were measured in serum samples of 167 ACA IgG+ SSc patients. Patients were divided in a SSc (fulfilling ACR 2013 criteria, n=132) and a very early SSc group (fulfilling VEDOSS criteria, n=35). Additionally, PBMCs from ACA IgG+ SSc patients (and ATA IgG + SSc and healthy donors (HD) as control) were cultured either in the presence of CD40L expressing fibroblasts, IL-21 and BAFF or without stimulation. Levels of ACA IgG, IgA and IgM (and total Ig) were measured after one week of culture using ELISA. Results ACA IgG+ SSc patients displayed a broad isot

Author

Xie, X; Delft, MV; Trouw, L; Conaghan, P; Ponchel, F

Publisher

BMJ Publishing Group Ltd and European League Against Rheumatism

Published

2019

Language

EN