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Simultaneous synthesis of 2-phenylethanol and L-homophenylalanine using aromatic transaminase with yeast Ehrlich pathway by Joon-Young Hwang; Jihyang Park; Joo-Hyun Seo; Minho Cha; Byung-Kwan Cho; Juhan Kim; Byung-Gee Kim is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Simultaneous synthesis of 2-phenylethanol and L-homophenylalanine using aromatic transaminase with yeast Ehrlich pathway about?
## Abstract 2‐Phenylethanol is a widely used aroma compound with rose‐like fragrance and L‐homophenylalanine is a building block of angiotensin‐converting enzyme (ACE) inhibitor. 2‐phenylethanol and L‐homophenylalanine were synthesized simultaneously with high yield from 2‐oxo‐4‐phenylbutyric acid and L‐phenylalanine, respectively. A recombinant __Escherichia coli__ harboring a coupled reaction pathway comprising of aromatic transaminase, phenylpyruvate decarboxylase, carbonyl reductase, and glucose dehydrogenase (GDH) was constructed. In the coupled reaction pathway, the transaminase reaction was coupled with the Ehrlich pathway of yeast; (1) a phenylpyruvate decarboxylase (YDR380W) as the enzyme to generate the substrate for the carbonyl reductase from phenylpyruvate (i.e., byproduct of the transaminase reaction) and to shift the reaction equilibrium of the transaminase reaction, and (2) a carbonyl reductase (YGL157W) to produce the 2‐phenylethanol. Selecting the right carbonyl reductase showing the highest activity on phenylacetaldehyde with narrow substrate specificity was the key to success of the constructing the coupling reaction. In addition, NADPH regeneration was achieved
Who reads Simultaneous synthesis of 2-phenylethanol and L-homophenylalanine using aromatic transaminase with yeast Ehrlich pathway?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Joon-Young Hwang; Jihyang Park; Joo-Hyun Seo; Minho Cha; Byung-Kwan Cho; Juhan Kim; Byung-Gee Kim
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)