Opening book details…
Can I read Influence of TNFA on the formation of liver metastases in a syngenic mouse model on EtoBox?
Influence of TNFA on the formation of liver metastases in a syngenic mouse model by Jörg W. Sturm; Richard Magdeburg; Kristine Berger; Beate Petruch; Stefan Samel; Roderich Bönninghoff; Michael Keese; Mattias Hafner; Stefan Post is a Medicine article available to read on EtoBox.
What is Influence of TNFA on the formation of liver metastases in a syngenic mouse model about?
## Abstract The level of TNFα expression is increased after partial hepatectomy, and experimental evidence exists that TNFα plays a key role in liver regeneration. Contradictory results are reported about the influence of TNFα on tumor growth: on the one hand, stimulation of tumor growth in various animal models and, on the other hand, intraperitoneally administered TNFα leads to reduced metastasis formation. TNFα may be one responsible factor for increased metastasis formation after surgical trauma. The objective of our study was to clarify the influence of TNFα on the formation of liver metastases in a syngenic mouse model __in vivo__. We used a novel marker system, EGFP transfected C26 tumor cells for __in vivo__ observation of metastasis formation by intravital microscopy. We analyzed the effect of intraperitoneal TNFα‐injection on tumor cell adhesion, extravasation and tumor development. The expression of ICAM‐1, VCAM‐1 and E‐Selectin was measured by Western blot and immunohistochemical staining. Tumor load was assessed by determining EGFP in Western blots. GdCl~3~ was employed 24 and 48 hr before tumor cell injection to selectively deplete the liver of functioning Kupffer cel
Who reads Influence of TNFA on the formation of liver metastases in a syngenic mouse model?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Jörg W. Sturm; Richard Magdeburg; Kristine Berger; Beate Petruch; Stefan Samel; Roderich Bönninghoff; Michael Keese; Mattias Hafner; Stefan Post
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)