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Can I read Macropinocytosis and Clathrin-Dependent Endocytosis Play Pivotal Roles for the Infectious Entry of Puumala Virus. on EtoBox?
Macropinocytosis and Clathrin-Dependent Endocytosis Play Pivotal Roles for the Infectious Entry of Puumala Virus. by Sandy Bauherr; Filip Larsberg; Annett Petrich; Hannah Sabeth Sperber; Victoria Klose-Grzelka; Madlen Luckner; Walid Azab; Matthias Schade; Chris Tina Höfer; Maik Joerg Lehmann; Peter T. Witkowski; Detlev H. Krüger; Andreas Herrmann; Roland Schwarzer is a Medicine article available to read on EtoBox.
What is Macropinocytosis and Clathrin-Dependent Endocytosis Play Pivotal Roles for the Infectious Entry of Puumala Virus. about?
Viruses from the family are encountered as emerging pathogens causing two life-threatening human zoonoses: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS), with case fatality rates of up to 50%. Here, we comprehensively investigated entry of the Old World hantavirus Puumala virus (PUUV) into mammalian cells, showing that upon treatment with pharmacological inhibitors of macropinocytosis and clathrin-mediated endocytosis, PUUV infections are greatly reduced. We demonstrate that the inhibitors did not interfere with viral replication and that RNA interference, targeting cellular mediators of macropinocytosis, decreases PUUV infection levels significantly. Moreover, we established lipophilic tracer staining of PUUV particles and show colocalization of stained virions and markers of macropinosomes. Finally, we report a significant increase in the fluid-phase uptake of cells infected with PUUV, indicative of a virus-triggered promotion of macropinocytosis. The family comprises a diverse group of virus species and is considered an emerging global public health threat. Individual hantavirus species differ considerably in terms of their pathogeni
Who reads Macropinocytosis and Clathrin-Dependent Endocytosis Play Pivotal Roles for the Infectious Entry of Puumala Virus.?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Sandy Bauherr; Filip Larsberg; Annett Petrich; Hannah Sabeth Sperber; Victoria Klose-Grzelka; Madlen Luckner; Walid Azab; Matthias Schade; Chris Tina Höfer; Maik Joerg Lehmann; Peter T. Witkowski; Detlev H. Krüger; Andreas Herrmann; Roland Schwarzer
- Publisher
- American Society for Microbiology (ISSN 0022-538X)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)