About this Biochemistry, Genetics and Molecular Biology article

Long-term stability of RNA in post-mortem bovine skeletal muscle, liver and subcutaneous adipose tissues by Bojlul Bahar; Frank J Monahan; Aidan P Moloney; Olaf Schmidt; David E MacHugh; Torres Sweeney is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Background Recovering high quality intact RNA from post-mortem tissue is of major concern for gene expression studies in animals and humans. Since the availability of post-mortem tissue is often associated with substantial delay, it is important that we understand the temporal variation in the stability of total RNA and of individual gene transcripts so as to be able to appropriately interpret the data generated from such studies. Hence, the objective of this experiment was to qualitatively and quantitatively assess the integrity of total and messenger RNA extracted from bovine skeletal muscle, subcutaneous adipose tissue and liver stored at 4°C at a range of time points up to 22 days post-mortem. These conditions were designed to mimic the environment prevailing during the transport of beef from the abattoir to retail outlets. ## Results The 28S and 18S rRNA molecules of total RNA were intact for up to 24 h post-mortem in liver and adipose tissues and up to 8 days post-mortem in skeletal muscle. The mRNA of housekeeping genes (__GAPDH__ and __ACTB__) and two diet-related genes (__RBP5__ and __SCD__) were detectable up to 22 days post-mortem in skeletal muscle. While the mRNA st

Author

Bojlul Bahar; Frank J Monahan; Aidan P Moloney; Olaf Schmidt; David E MacHugh; Torres Sweeney

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)