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Regulation of XBP‐1 signaling during transient and stable recombinant protein production in CHO cells by Sebastian C. Y. Ku; Poh Choo Toh; Yih Yean Lee; Janet Chusainow; Miranda G. S. Yap; Sheng‐Hao Chao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Regulation of XBP‐1 signaling during transient and stable recombinant protein production in CHO cells about?
## Abstract X‐box binding protein 1 (XBP‐1) is a key regulator of cellular unfolded protein response (UPR). The spliced isoform of XBP‐1, XBP‐1S, is a transcription activator, which is expressed only when UPR is induced. However, the impact of recombinant protein production on the regulation of XBP‐1 signaling in CHO cells is not well understood. In this report, we cloned the Chinese hamster XBP‐1 homolog to aid the investigation of the interplay between protein productivity, culture conditions, and endogenous XBP‐1 signaling in CHO cells. Interestingly, expression of XBP‐1S is detected in the non‐producing and unstressed CHO‐K1 cells. Transient expression of recombinant erythropoietin reveals a positive correlation between XBP‐1 mRNA abundance and protein production level. However, such a correlation is not observed in batch cultivation of stable producing cell lines. The increased XBP‐1 splicing is detected in late‐phase cultures, suggesting that induction of XBP‐1S may be a result of nutrient limitations or other environmental stresses rather than that of increased intracellular accumulation of recombinant proteins. Our data suggest that XBP‐1 is a key determinant for the secret
Who reads Regulation of XBP‐1 signaling during transient and stable recombinant protein production in CHO cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sebastian C. Y. Ku; Poh Choo Toh; Yih Yean Lee; Janet Chusainow; Miranda G. S. Yap; Sheng‐Hao Chao
- Publisher
- American Institute of Chemical Engineers; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley; American Chemical Society (ACS) (ISSN 8756-7938)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)