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The document summarizes a study characterizing the Egyptian strain of bovine viral diarrhea virus (BVDV) called Iman. A nested polymerase chain reaction assay was used to identify the strain as BVDV type 1. The Iman strain was propagated in cell culture for 16 passages and caused cytopathic effects including cell rounding and vacuolation. Reverse transcription PCR detected viral RNA in cell-associated harvests starting at 4 hours post infection and in cell-free harvests at 8 hours, indicating RNA synthesis

Author

Ghada Afifi

Language

EN