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Estrogen Treatment of Spinal Cord Injury Attenuates Calpain Activation and Apoptosis by Eric Anthony Sribnick; Deborah Denise Matzelle; Swapan Kumar Ray; Naren Lal Banik is a Neuroscience article available to read on EtoBox.

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## Abstract Spinal cord injury (SCI) is a devastating neurologic injury, and currently, the only recommended pharmacotherapy is high‐dose methylprednisolone, which has limited efficacy. Estrogen is a multi‐active steroid with anti‐oxidant and anti‐apoptotic effects. Estrogen may modulate intracellular Ca^2+^ and prevent inflammation. For this study, male rats were divided into three groups. Sham‐group animals received a laminectomy at T12. Injured rats received both laminectomy and 40 gram centimeter force SCI. Estrogen‐group rats received 4 mg/kg 17β‐estradiol (estrogen) at 15 min and 24 hr post‐injury, and vehicle‐group rats received equal volumes of dimethyl sulfoxide. Animals were sacrificed at 48 hr post‐injury, and 1‐cm segments of the lesion, rostral penumbra, and caudal penumbra were excised. The degradation of 68 kD neurofilament protein (NFP) and estrogen receptors (ER) was examined by Western blot analysis. Protein levels of calpain and the activities of calpain and caspase‐3 were also examined. Levels of cytochrome c were determined in both cytosolic and mitochondrial fractions. Cell death with DNA fragmentation was examined using the TUNEL assay. At the lesion, samples

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Author

Eric Anthony Sribnick; Deborah Denise Matzelle; Swapan Kumar Ray; Naren Lal Banik

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2006

Language

EN

Field

Neuroscience (Life Sciences)