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Ozone exposure and blood transcriptome: A randomized, controlled, crossover trial among healthy adults by Xihao Du; Yue Niu; Cuiping Wang; Weidong Wang; Cong Liu; Xia Meng; Chen Chu; Renjie Chen; Haidong Kan is a Environmental Science article available to read on EtoBox.

What is Ozone exposure and blood transcriptome: A randomized, controlled, crossover trial among healthy adults about?

## Rationale: Transcriptome-wide analysis is powerful in studying systemic rna changes following environmental exposures. however, impacts of ozone inhalation on circulating transcriptome have not yet been examined. ## Objectives: To explore the impact of acute ozone exposure on circulating transcriptome using rna sequencing (rna-seq). ## Methods: We recruited 32 healthy young adults in a randomized, crossover, controlled exposure trial. each participant completed two 2-h exposure sessions of ozone (200 ppb) and clean air, respectively. blood samples were collected at the end of each session and were used for rna-seq. the differentially expressed genes associated with ozone exposure were assessed using bayesian adjusted statistics from linear models in the limma r package. ## Results: A total of 29 participants finished this trial and donated their blood samples for transcriptome analysis. the average concentration of ozone was 7.8 ± 2.6 ppb under clean air and 201.1 ± 1.7 ppb under ozone exposure session. a total of 1899 genes were significantly changed (1067 up-regulated and 832 down-regulated) by ozone exposure at a false discovery rate 1.2 or < 0.8. the top 10 terms of biolog

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Author

Xihao Du; Yue Niu; Cuiping Wang; Weidong Wang; Cong Liu; Xia Meng; Chen Chu; Renjie Chen; Haidong Kan

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Environmental Science (Physical Sciences)