Can I read Parathyroid hormone‐induced changes in alkaline phosphatase expression in fetal calvarial osteoblasts: Differences between rat and mouse on EtoBox?

Parathyroid hormone‐induced changes in alkaline phosphatase expression in fetal calvarial osteoblasts: Differences between rat and mouse by J. W. J. M. Jongen; M. P. Bos; J. M. Van Der Meer; M. P. M. Herrmann‐Erlee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Parathyroid hormone‐induced changes in alkaline phosphatase expression in fetal calvarial osteoblasts: Differences between rat and mouse about?

We studied the effects of parathyroid hormone (PTH) o n two markers of the osteoblast phenotype: alkaline phosphatase (AP) (activity and mRNA) and cyclic adenosine monophosphate (CAMP) accumulation. Osteoblast-like cells derived from fetal rat (ROB) and mouse (MOB) calvariae were isolated by collagenase treatment. Cells were cultured in a-Minimal Essential Medium (MEM) with 2% fetal calf serum (FCS) for 4 days. In ROB and MOB bPTH(1-34) induced a fast increase (up to 5 minutes) in cAMP accumulation. When equal amounts of cells were seeded, the cAMP accumulation was higher in MOB than in ROB. No difference in basal AP activity was observed between ROB and MOB. When bPTH(1-34) was added to ROB for the last 24 or 48 hr, AP activity decreased dose dependently. However, MOB treated with bPTH(1-34) for the last 24 or 48 hours showed an increase of AP activity. Basal AP activity was positively correlated with the seeding density of ROB and MOB cultures. Basal AP activity influenced the degree of inhibition (ROB) or stimulation (MOB) after incubation with bPTH(1-34).

Who reads Parathyroid hormone‐induced changes in alkaline phosphatase expression in fetal calvarial osteoblasts: Differences between rat and mouse?

Author

J. W. J. M. Jongen; M. P. Bos; J. M. Van Der Meer; M. P. M. Herrmann‐Erlee

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)