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Lentiviral vector integration sites in human NOD/SCID repopulating cells by Stephanie Laufs; Guillermo Guenechea; Africa Gonzalez‐Murillo; K. Zsuzsanna Nagy; M. Luz Lozano; Coral del Val; Sunitha Jonnakuty; Agnes Hotz‐Wagenblatt; W. Jens Zeller; Juan A. Bueren; Stefan Fruehauf is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Lentiviral vector integration sites in human NOD/SCID repopulating cells about?
## Abstract ## Background Recent observations of insertional mutagenesis in preclinical and clinical settings emphasize the relevance of investigating comprehensively the spectrum of integration sites targeted by specific vectors. ## Methods We followed the engraftment of lentivirally transduced human cord blood (CB) progenitor cells after transplantation into NOD/SCID mice using a self‐inactivating HIV‐1‐derived vector expressing the enhanced green fluorescent protein (EGFP). ## Results The mean of transduction of CD34^+^ CB cells was 41%, as deduced from the percentage of EGFP^+^ cells before transplantation. At 3 weeks post‐transplantation, the average of EGFP^+^ cells in the human cell population was 65 ± 8%, and increased to 75 ± 10% at 12 weeks post‐transplantation. In order to determine the proviral integration sites in human NOD/SCID repopulating cells (SRCs) we used the ligation‐mediated polymerase chain reaction (LM‐PCR) technique. Sixty‐eight percent of the integrations were found to be located in RefSeq genes, most of them in intron regions. Twenty percent of these integrations occurred within a distance of 10 kb from the transcription start site; a percentage that is s
Who reads Lentiviral vector integration sites in human NOD/SCID repopulating cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Stephanie Laufs; Guillermo Guenechea; Africa Gonzalez‐Murillo; K. Zsuzsanna Nagy; M. Luz Lozano; Coral del Val; Sunitha Jonnakuty; Agnes Hotz‐Wagenblatt; W. Jens Zeller; Juan A. Bueren; Stefan Fruehauf
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)