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Effect of cAMP derivates on assembly and maintenance of tight junctions in human umbilical vein endothelial cells by Michaela Beese; Kristin Wyss; Marion Haubitz; Torsten Kirsch is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Background Endothelial tight and adherens junctions control a variety of physiological processes like adhesion, paracellular transport of solutes or trafficking of activated leukocytes. Formation and maintenance of endothelial junctions largely depend on the microenvironment of the specific vascular bed and on interactions of the endothelium with adjacent cell types. Consequently, primary cultures of endothelial cells often lose their specific junctional pattern and fail to establish tight monolayer __in vitro__. This is also true for endothelial cells isolated from the vein of human umbilical cords (HUVEC) which are widely used as model for endothelial cell-related studies. ## Results We here compared the effect of cyclic 3'-5'-adenosine monophosphate (cAMP) and its derivates on formation and stabilization of tight junctions and on alterations in paracellular permeability in HUVEC. We demonstrated by light and confocal laser microscopy that for shorter time periods the sodium salt of 8-bromoadenosine-cAMP (8-Br-cAMP/Na) and for longer incubation periods 8-(4-chlorophenylthio)-cAMP (pCPT-cAMP) exerted the greatest effects of all compounds tested here on formation of

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Author

Michaela Beese; Kristin Wyss; Marion Haubitz; Torsten Kirsch

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-2018.; Springer Science and Business Media LLC (ISSN 1471-2121)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)