About this Biochemistry, Genetics and Molecular Biology article

Riboproteomic analysis of polypeptides interacting with the internal ribosome-entry site element of foot-and-mouth disease viral RNA by Almudena Pacheco; Sandrine Reigadas; Encarnación Martínez-Salas is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Abstract Initiation of translation driven by internal ribosome entry site (IRES) elements depends upon the structural organization of this mRNA region. Besides translation initiation factors (eIFs), auxiliary proteins can also affect IRES activity. With the aim to identify proteins interacting with two unrelated IRESs present in the genome of foot‐and‐mouth disease virus (FMDV) and hepatitis C virus (HCV) we have used a proteomic approach. This procedure allowed the identification of 21 RNA‐binding proteins interacting with discrete regions of the FMDV IRES, domains 3 and 5, and 16 interacting with domain III of the HCV IRES. In support of the binding specificity, the factors interacting with domain 3 differed from those interacting with domain 5, and included three poly(rC)‐binding protein (PCBP) members, besides proliferation‐associated 2G4 (PA2G4) and deleted‐azoospermia 1 (DAZ1) protein. Around 71% of the identified factors associated with the FMDV IRES differ from those interacting with the HCV IRES. The group of proteins interacting with the FMDV or the HCV IRES includes eIF4B and 5 subunits of eIF3, respectively, known to interact with each of these RNAs, validating the r

Author

Almudena Pacheco; Sandrine Reigadas; Encarnación Martínez-Salas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)