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Can I read Iridoid glycoside-based quantitative chromatographic fingerprint analysis: A rational approach for quality assessment of Indian medicinal plant Gambhari (Gmelina arborea) on EtoBox?
Iridoid glycoside-based quantitative chromatographic fingerprint analysis: A rational approach for quality assessment of Indian medicinal plant Gambhari (Gmelina arborea) by Akhilesh K. Yadav; N. Tiwari; P. Srivastava; Subhash C. Singh; K. Shanker; Ram K. Verma; Madan M. Gupta is a Chemistry article available to read on EtoBox.
What is Iridoid glycoside-based quantitative chromatographic fingerprint analysis: A rational approach for quality assessment of Indian medicinal plant Gambhari (Gmelina arborea) about?
A sensitive, selective and robust qualitative and quantitative densitometric high-performance thin layer chromatographic method was developed and validated for the determination of iridoid glycoside in the aerial part of Gambhari (Gmelina arborea). Iridoid gycoside 6-O-(2 ,3 -dibenzoyl)-␣-l-rhamnopyranosylcatalpol (IG) was used as a chemical marker for the standardization of G. arborea plant extracts. The separation was performed on aluminum Kieselgel 60F 254 TLC plates using chloroform-methanol as mobile phase. The quantitation of IG was carried out using the densitometric reflection/absorption mode at 240 and 430 nm after post-chromatographic derivatization with vanillin-sulphuric acid reagent. A precise and accurate quantification can be performed in the linear working concentration range of 1000-5000 ng/spot with good correlation (r 2 = 0.994). The method was validated for peak purities, precision, robustness, limit of detection (LOD) and quantitation (LOQ), etc., as per ICH guidelines. Specificity of quantitation was confirmed using retention factor (R f ), UV-vis spectral correlation and ESI-MS spectra of marker compound (IG) in sample track.
Who reads Iridoid glycoside-based quantitative chromatographic fingerprint analysis: A rational approach for quality assessment of Indian medicinal plant Gambhari (Gmelina arborea)?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Akhilesh K. Yadav; N. Tiwari; P. Srivastava; Subhash C. Singh; K. Shanker; Ram K. Verma; Madan M. Gupta
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)
- Published
- 2008
- Language
- EN
- Field
- Chemistry (Physical Sciences)