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Zonula Occludens-1 Demonstrates a Unique Appearance in Buccal Mucosa Over Several Layers by Keisuke Imafuku; Mayumi Kamaguchi; Ken Natsuga; Hideki Nakamura; Hiroshi Shimizu; Hiroaki Iwata is a Neuroscience article available to read on EtoBox.

What is Zonula Occludens-1 Demonstrates a Unique Appearance in Buccal Mucosa Over Several Layers about?

Tight junctions (TJs) firmly seal epithelial cells and are key players in the epithelial barrier. TJs consist of several proteins, including those of the transmembrane claudin family and the scaffold zonula occludens (ZO) family. Epithelial tissues are exposed to different conditions: to air in the stratified epithelium of the skin and to liquids in the monolayer of the intestine. The TJs in stratified oral mucosal epithelium have remained insufficiently elucidated in terms of distributions, appearances and barrier functions of TJ proteins in normal buccal mucosa. We investigated these and ZO-1 and claudin-1 were found to be expressed in the top third and in the bottom three quarters of the mucosal epithelium. ZO-1 in the buccal mucosa was found to have an irregular linear appearance. ZO-1 in the buccal mucosa continuously existed in several layers. Electron microscopy revealed the buccal mucosa to have kissing points. In a biotin permeation assay that sought to investigate inside-outside barrier function, the biotin tracer penetrated several ZO-1 layers but did not pass through all the ZO-1 layers. We found that the oral mucosal cell knockdown of TJP1 or CLDN1 resulted in decrease

Who reads Zonula Occludens-1 Demonstrates a Unique Appearance in Buccal Mucosa Over Several Layers?

Author

Keisuke Imafuku; Mayumi Kamaguchi; Ken Natsuga; Hideki Nakamura; Hiroshi Shimizu; Hiroaki Iwata

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Neuroscience (Life Sciences)