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CRFergic Innervation Of the Paraventricular Nucleus of the Rat Hypothalamus: A Tract-Tracing Study by Danielle Champagne; Johanne Beaulieu; Guy Drolet is a Neuroscience article available to read on EtoBox.

What is CRFergic Innervation Of the Paraventricular Nucleus of the Rat Hypothalamus: A Tract-Tracing Study about?

It has been reported that corticotropin‐releasing factor (CRF) may regulate its own biosynthesis in the paraventricular nucleus of the hypothalamus (PVH). Whether this CRF autoregulation is mediated by local circuitry or from extra‐PVH CRF neuronal fibers terminating on CRF perikarya within the PVH is unknown. In the present study, we sought to determine the origin(s) of this CRF innervation using retrograde transport of wheat germ‐conjugated‐gold particles (WGA‐apoHRP‐Au) combined with immunohistochemistry for CRF. The rats also received colchicine (100 μg, icv) 5–7 days after tracer injection and were perfused 24 h later. Results of retrograde labeling with pressure injections of WGA‐apoHRP‐Au centered to PVH and subsequent immunohistochemical staining for CRF demonstrated numerous retrogradely labeled CRF neurons in the perifornical hypothalamic nucleus (PeF), the dorsolateral hypothalamic area (DA) (medial and lateral portions) and the dorsomedial nucleus of the hypothalamus (DMH). Smaller groups of CRF‐ir neurons that were retrogradely labeled were found in the bed nuclei of the stria terminalis (BnST), the Barrington’s nucleus (Bar) and the dorsal raphé (DR). These CRFergic p

Who reads CRFergic Innervation Of the Paraventricular Nucleus of the Rat Hypothalamus: A Tract-Tracing Study?

Author

Danielle Champagne; Johanne Beaulieu; Guy Drolet

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-8194)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)