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Evaluation of a Predictive Kinetic Procedure for the Enzymatic Measurement of Creatinine in Serum by Martín Yago; María José Olmos; Trinidad Gómez is a Medicine article available to read on EtoBox.

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## Abstract We have evaluated the analytical performance of a predictive kinetic procedure for the enzymatic measurement of serum creatinine in comparison with equilibrium and kinetic fixed-time procedures. The procedure uses commercially available reagents and is based on calculating the total change in absorbance expected if the reaction were to proceed to equilibrium by fitting the initial part of the absorbance-time curve to a three-parameter function by non-linear regression. Total imprecision for different concentrations of creatinine ranges from 1.47 to 1.86% and is significantly better than that obtained for the fixed-time procedure. The results with the predictive procedure are less dependent on creatinine amidohydrolase activity in the reagent, pH of the reagent and temperature of analysis than are the results with the fixed-time procedure. All the procedures are interfered with by bilirubin and ascorbic acid to about the same extent. Recovery and linearity are quite acceptable, and the estimated accuracy is below 2.1%. However, in comparison with the equilibrium procedure, the predictive procedure tends to underestimate creatinine concentrations at values below 100 μmol/

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Author

Martín Yago; María José Olmos; Trinidad Gómez

Publisher

Walter de Gruyter GmbH

Published

1998

Language

EN

Field

Medicine (Health Sciences)