Can I read Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions on EtoBox?

Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions by Tingting Jiang; Ramya Raviram; Valentina Snetkova; Pedro P. Rocha; Charlotte Proudhon; Sana Badri; Richard Bonneau; Jane A. Skok; Yuval Kluger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions about?

Use of low resolution single cell DNA FISH and population based high resolution chromosome conformation capture techniques have highlighted the importance of pairwise chromatin interactions in gene regulation. However, it is unlikely that associations involving regulatory elements act in isolation of other interacting partners that also influence their impact. Indeed, the influence of multi-loci interactions remains something of an enigma as beyond low-resolution DNA FISH we do not have the appropriate tools to analyze these. Here we present a method that uses standard 4C-seq data to identify multi-loci interactions from the same cell. We demonstrate the feasibility of our method using 4C-seq data sets that identify known pairwise and novel tri-loci interactions involving the __Tcrb__ and __Igk__ antigen receptor enhancers. We further show that the three __Igk__ enhancers, MiEκ, 3′Eκ and Edκ, interact simultaneously in this super-enhancer cluster, which add to our previous findings showing that loss of one element decreases interactions between all three elements as well as reducing their transcriptional output. These findings underscore the functional importance of simultaneous in

Who reads Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions?

Author

Tingting Jiang; Ramya Raviram; Valentina Snetkova; Pedro P. Rocha; Charlotte Proudhon; Sana Badri; Richard Bonneau; Jane A. Skok; Yuval Kluger

Publisher

Oxford University Press (OUP)

Published

2016

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)