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The structure of the lipopolysaccharide from a galU mutant of Pseudomonas aeruginosa serogroup-O11 by Biswa Choudhury; Russell W. Carlson; Joanna B. Goldberg is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The lipopolysaccharide (LPS) of a galU mutant of Pseudomonas aeruginosa PA103, a serogroup O11 strain, was sequentially extracted with phenol-chloroform-petroleum ether (PCP) followed by hot phenol-water extraction of the bacterial pellet remaining after PCP extraction. LPS was found in both the PCP extract as well as in the water phase of the hot phenol-water extract. Analysis of the carbohydrate portion released by mild acid hydrolysis of both LPS preparations, both before and after removal of all phosphate groups by treatment with aqueous HF, was performed by glycosyl composition and linkage analyses as well as by NMR and mass spectrometric analyses. The results showed that the carbohydrate portion of these two LPS extracts contained the same structure: namely, a-GalN(Ala)-(1!3)-a-(7-Cm)HepII-(1!3)-a-HepI-(1!5)-a-Kdo-(2!. The oligosaccharide preparation from PCP-extracted LPS consisted of a variety of structures containing up to six phosphate groups present as mono-, pyro-, and possibly triphosphate, primarily located on the HepI residue with some molecules having a monophosphate on HepII. The oligosaccharide preparation from the hot phenol-water-extracted LPS contained a simila

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Author

Biswa Choudhury; Russell W. Carlson; Joanna B. Goldberg

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)