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Blakeslea trispora Photoreceptors: Identification and Functional Analysis by Wei Luo; Chao Xue; Yuzheng Zhao; Huili Zhang; Zhiming Rao; Xiaobin Yu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Blakeslea trispora Photoreceptors: Identification and Functional Analysis about?

is an industrial fungal species used for large-scale production of carotenoids. However, light-regulated physiological processes, such as carotenoid biosynthesis and phototropism, are not fully understood. In this study, we isolated and characterized three photoreceptor genes, , , and , in Bioinformatics analyses of these genes and their protein sequences revealed that the functional domains (PAS/LOV [Per-ARNT-Sim/light-oxygen-voltage] domain and zinc finger structure) of the proteins have significant homology to those of other fungal blue-light regulator proteins expressed by and The photoreceptor proteins were synthesized by heterologous expression in The chromogenic groups consisting of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) were detected to accompany BTWC-1 proteins by using high-performance liquid chromatography (HPLC) and fluorescence spectrometry, demonstrating that the proteins may be photosensitive. The absorbance changes of the purified BTWC-1 proteins seen under dark and light conditions indicated that they were light responsive and underwent a characteristic photocycle by light induction. Site-directed mutagenesis of the cysteine residual (Cys

Who reads Blakeslea trispora Photoreceptors: Identification and Functional Analysis?

Author

Wei Luo; Chao Xue; Yuzheng Zhao; Huili Zhang; Zhiming Rao; Xiaobin Yu

Publisher

American Society for Microbiology

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)