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Can I read An immunotoxin containing a rat IgM monoclonal antibody (Campath 1) and saporin 6: effect on T lymphocytes and hemopoietic cells on EtoBox?
An immunotoxin containing a rat IgM monoclonal antibody (Campath 1) and saporin 6: effect on T lymphocytes and hemopoietic cells by Pier Luigi Tazzari; Luigi Barbieri; Marco Gobbi; Angelo Dinota; Simonetta Rizzi; Andrea Bontadini; Annalisa Pession; Sante Tura; Fiorenzo Stirpe is a Immunology and Microbiology article available to read on EtoBox.
What is An immunotoxin containing a rat IgM monoclonal antibody (Campath 1) and saporin 6: effect on T lymphocytes and hemopoietic cells about?
The elimination of the cells responsible for graft-versus-host disease in allogeneic bone marrow transplantation has been attempted with a variety of methods, including the use of the ribosome-inactivating toxin ricin bound to monoclonal antibodies acting as carriers. However the high nonspecific toxicity of these immunotoxins containing the whole toxin greatly limited clinical application. Toxicity can be reduced using the A-chain of ricin or other ribosome-inactivating proteins (RIPs) which are devoid of a B-chain with lectin properties. We used saporin 6 purified from Saponaria officinalis seeds, which was conjugated with the rat IgM monoclonal antibody Campath 1 specific for mature T and B lymphocytes as well as for monocytes. The immunotoxin retained both RIP and antibody activity, inhibiting protein synthesis both in a cell-free system and in cells bearing the Campath 1 antigen; it also abolished methyl 3H-thymidine uptake in phytohemagglutinin-stimulated T lymphocytes. Myeloid progenitors were largely spared as shown by myeloid stem cell (CFU-GM) growth which was scarcely affected. Toxicity of the immunotoxin to cell lines not expressing the antigen recognized by Campath 1 m
Who reads An immunotoxin containing a rat IgM monoclonal antibody (Campath 1) and saporin 6: effect on T lymphocytes and hemopoietic cells?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Pier Luigi Tazzari; Luigi Barbieri; Marco Gobbi; Angelo Dinota; Simonetta Rizzi; Andrea Bontadini; Annalisa Pession; Sante Tura; Fiorenzo Stirpe
- Publisher
- Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0340-7004)
- Published
- 1987
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)