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Sialylation of Integrin β1 is Involved in Radiation-Induced Adhesion and Migration in Human Colon Cancer Cells by Minyoung Lee; Hae-June Lee; Woo Duck Seo; Ki Hun Park; Yun-Sil Lee is a Medicine article available to read on EtoBox.

What is Sialylation of Integrin β1 is Involved in Radiation-Induced Adhesion and Migration in Human Colon Cancer Cells about?

Purpose: Previously, we reported that radiation-induced ST6 Gal I gene expression was responsible for an increase of integrin b1 sialylation. In this study, we have further investigated the function of radiation-mediated integrin b1 sialylation in colon cancer cells. Methods and Materials: We performed Western blotting and lectin affinity assay to analyze the expression and level of sialylated integrin b1. After exposure to ionizing radiation (IR), adhesion and migration of cells were measured by in vitro adhesion and migration assay. Results: IR increased sialylation of integrin b1 responsible for its increased protein stability and adhesion and migration of colon cancer cells. However, for cells with an N-glycosylation site mutant of integrin b1 located on the I-like domain (Mu3), these effects were dramatically inhibited. In addition, integrin b1-mediated radioresistance was not observed in cells containing this mutant. When sialylation of integrin b1 was targeted with a sulfonamide chalcone compound, inhibition of radiation-induced sialylation of integrin b1 and inhibition of radiation-induced adhesion and migration occurred. Conclusion: The increase of integrin b1 sialylation

Who reads Sialylation of Integrin β1 is Involved in Radiation-Induced Adhesion and Migration in Human Colon Cancer Cells?

Author

Minyoung Lee; Hae-June Lee; Woo Duck Seo; Ki Hun Park; Yun-Sil Lee

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0360-3016)

Published

2010

Language

EN

Field

Medicine (Health Sciences)