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Mitochondrial Voltage‐dependent Anion Channel Is Involved in Dopamine‐induced Apoptosis by A. Premkumar; R. Simantov is a Neuroscience article available to read on EtoBox.

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## Abstract Neuronal NMB cells were used to determine changes in gene expression upon treatment with dopamine. Twelve differentially expressed cDNAs were identified and cloned, one of them having 99.4% sequence homology with isoform 2 of a voltage‐dependent anion channel (VDAC‐2). The known role of VDAC, a mitochondrial outer‐membrane protein, in transport of anions, pore formation, and release of cytochrome C prompted us to investigate the possible role of VDAC gene family in dopamine‐induced apoptosis. Semi‐quantitative PCR analysis indicated that expression of the three VDAC isoforms was reduced by dopamine. Immunoblotting with anti‐VDAC antibodies detected two VDAC protein bands of 33 and 34 kDa. Dopamine decreased differentially the immunoreactivity of the 34 kDa protein. Whether the decrease in VDAC expression influence the mitochondrial membrane potential (ΔΨm) was determined with the dye Rhodamine‐123. Dopamine indeed decreased the mitochondrial ΔΨm, but the maximum effect was observed within 3 h, prior to the decrease in VDAC mRNA or protein levels. Cyclosporin A, a blocker of the mitochondrial pore complex, prevented the decrease in ΔΨm, but did not rescue the cells from

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Author

A. Premkumar; R. Simantov

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)