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Can I read Molecular Conservation of Estrogen-response Associated with Cell Cycle Regulation, Hormonal Carcinogenesis and Cancer in Zebrafish and Human Cancer Cell Lines on EtoBox?
Molecular Conservation of Estrogen-response Associated with Cell Cycle Regulation, Hormonal Carcinogenesis and Cancer in Zebrafish and Human Cancer Cell Lines by Siew Hong Lam; Serene GP Lee; Chin Y Lin; Jane S Thomsen; Pan Y Fu; Karuturi RK Murthy; Haixia Li; Kunde R Govindarajan; Lin CH Nick; Guillaume Bourque; Zhiyuan Gong; Thomas Lufkin; Edison T Liu; Sinnakaruppan Mathavan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Molecular Conservation of Estrogen-response Associated with Cell Cycle Regulation, Hormonal Carcinogenesis and Cancer in Zebrafish and Human Cancer Cell Lines about?
## Background The zebrafish is recognized as a versatile cancer and drug screening model. However, it is not known whether the estrogen-responsive genes and signaling pathways that are involved in estrogen-dependent carcinogenesis and human cancer are operating in zebrafish. In order to determine the potential of zebrafish model for estrogen-related cancer research, we investigated the molecular conservation of estrogen responses operating in both zebrafish and human cancer cell lines. ## Methods Microarray experiment was performed on zebrafish exposed to estrogen (17β-estradiol; a classified carcinogen) and an anti-estrogen (ICI 182,780). Zebrafish estrogen-responsive genes sensitive to both estrogen and anti-estrogen were identified and validated using real-time PCR. Human homolog mapping and knowledge-based data mining were performed on zebrafish estrogen responsive genes followed by estrogen receptor binding site analysis and comparative transcriptome analysis with estrogen-responsive human cancer cell lines (MCF7, T47D and Ishikawa). ## Results Our transcriptome analysis captured multiple estrogen-responsive genes and signaling pathways that increased cell proliferation, promo
Who reads Molecular Conservation of Estrogen-response Associated with Cell Cycle Regulation, Hormonal Carcinogenesis and Cancer in Zebrafish and Human Cancer Cell Lines?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Siew Hong Lam; Serene GP Lee; Chin Y Lin; Jane S Thomsen; Pan Y Fu; Karuturi RK Murthy; Haixia Li; Kunde R Govindarajan; Lin CH Nick; Guillaume Bourque; Zhiyuan Gong; Thomas Lufkin; Edison T Liu; Sinnakaruppan Mathavan
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2008]-; Springer Science and Business Media LLC (ISSN 1755-8794)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)