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In vitro permeability of eight β-blockers through Caco-2 monolayers utilizing liquid chromatography/electrospray ionization mass spectrometry by Caldwell, Gary W.; Easlick, Scott M.; Gunnet, Joseph; Masucci, John A.; Demarest, Keith is a Chemistry article available to read on EtoBox.
What is In vitro permeability of eight β-blockers through Caco-2 monolayers utilizing liquid chromatography/electrospray ionization mass spectrometry about?
It is demonstrated that the apparent permeability coefficients of b-adrenoceptor antagonist drugs can easily (P app ) be determined for Caco-2 cell culture intestinal models utilizing liquid chromatography/mass spectrometry (LC/MS). The LC/MS method with electrospray ionization in the single ion monitoring mode showed an increased sensitivity of 1000-fold compared with LC/UV detection and enhanced selectivity with respect to both LC/UV and radioactivity assays. The coefficients of b-adrenoceptor antagonists determined by LC/MS have the same P app ranking order as those determined by LC/UV and radioactivity assays. However, the coefficients determined P app in this study showed signiÐcant discrepancies from those determined in other laboratories. There are several experimental factors that directly a †ect the absolute value of the coefficients, including pH gradients, additional P app di †usion barriers (i.e. unstirred water layer and type of Ðlter support), analyte concentration, detection method and possibly cell culture variations. These parameters should be controlled when generating Caco-2 coefficients for P app di †erent compounds.
Who reads In vitro permeability of eight β-blockers through Caco-2 monolayers utilizing liquid chromatography/electrospray ionization mass spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Caldwell, Gary W.; Easlick, Scott M.; Gunnet, Joseph; Masucci, John A.; Demarest, Keith
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)
- Published
- 1998
- Language
- EN
- Field
- Chemistry (Physical Sciences)