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NF-κB Activation at Implantation Window of the Mouse Uterus by Hitomi Nakamura; Tadashi Kimura; Kazuhide Ogita; Takafumi Nakamura; Masahiko Takemura; Koichiro Shimoya; Sinsuke Koyama; Tomoko Tsujie; Masayasu Koyama; Yuji Murata is a Medicine article available to read on EtoBox.

What is NF-κB Activation at Implantation Window of the Mouse Uterus about?

**Problem:** Nuclear factor kappa B (NF‐__κ__B) is one candidate transcriptional modulator, which might regulate many kinds of molecules that play sequential roles at implantation in the endometrium. However, temporal and spatial activation of NF‐__κ__B at implantation window is unknown. **Methods:** Activation of NF‐__κ__B in the mouse uterus was determined by electrophoretic mobility shift assays. Localization of p50 and p65, components of NF‐__κ__B, was analyzed by immunohistochemistry. **Results:** NF‐__κ__B was activated in the proestrus and estrus phases in non‐pregnant uterus. In the pregnant uterus, NF‐__κ__B was activated after day 1.5 post‐coitum, and the activation continued during implantation period. The immunoreactivities of p50 and p65 were mainly localized in endometrial epithelium, and were weaker in endometrial stroma cells. **Conclusion:** NF‐__κ__B activity is dynamically regulated during the sexual cycle as well as during the implantation period in the endometrium, where the biochemical interaction between mother and conceptus first occurs.

Who reads NF-κB Activation at Implantation Window of the Mouse Uterus?

Author

Hitomi Nakamura; Tadashi Kimura; Kazuhide Ogita; Takafumi Nakamura; Masahiko Takemura; Koichiro Shimoya; Sinsuke Koyama; Tomoko Tsujie; Masayasu Koyama; Yuji Murata

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 8755-8920)

Published

2004

Language

EN

Field

Medicine (Health Sciences)