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Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations by Kenneth N. Kreuzer; Kevin McEntee; Adam P. Geballe; Nicholas R. Cozzarelli is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Defective lambda transducing phages for the nalA region of the Escherichia coli chromosome were isolated from a lysogen in which lambda is inserted in the nearby glpT gene. The three classes of transducing phages designated lambdanrdA, lambdaubiG, and lambdadnalA contained bacterial DNA extending from glpT through nrdA, ubiG, and nalA, respectively. The bacterial genes are in the left arm of the lambda chromosome. Of the eleven polypeptides coded by lambdadnalA that were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate only one was not also specified by lambdadubiG. This 105,000 dalton polypeptide is the nalA gene product. The electorphoretic mobility and isoelectric point of this protein were unaffected by a nalA mutation (nalA48) that confers nalidixic acid resistance. Temperature-sensitive and amber mutations in the nalA gene were isolated using a lambdadnalA48 lysogen which is heterodiploid for nalA. The conditional lethality of these mutations proves that nalA is an essential locus.

Who reads Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations?

Author

Kenneth N. Kreuzer; Kevin McEntee; Adam P. Geballe; Nicholas R. Cozzarelli

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1978

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)