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PARP Inhibitors: GST Assay and Ergosterol Analysis by lacerisk0 is a document available to read on EtoBox.
The document discusses methods for analyzing PARP inhibitors like Pimasertib and PJ34. It describes preparing protein extracts from cells treated with these inhibitors and validating the data. It also details measuring glutathione S-transferase (GST) activity using a GST assay kit to detect conjugation of glutathione with 1-chloro-2,4-dinitrobenzene, producing a product that absorbs light at 340nm. The activity is proportional to GST levels in the sample. Sterol quantification is also described to measure e
- Author
- lacerisk0
- Language
- EN