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What is Purif Cit C Ox R Sphaeroides - FEBS Letters (1995) about?

A rapid and efficient method for purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides using Ni2+-NTA affinity chromatography is presented. The method utilizes a six-histidine tag fused to subunit I, allowing for high yield and purity (> 95%) of the enzyme in a single step. This purification technique enhances the availability of both wildtype and mutant variants for further biochemical and biophysical studies.

Author

franco.castillo1965

Language

EN