About this document
Production of Human Somatostatin by GABRIELA NORMA SOLANO CANCHAYA is a document available to read on EtoBox.
The document describes the construction of an expression vector for producing and purifying human somatostatin in E. coli. Specifically, it details the creation of a new pGB-SMT plasmid to produce somatostatin as a fusion protein with Kluyveromyces lactis β-galactosidase, allowing IPTG-free expression and purification by CNBr cleavage and ion exchange chromatography. The suitability of this system was demonstrated at a 15L scale, yielding almost 300mg of highly pure somatostatin.
- Author
- GABRIELA NORMA SOLANO CANCHAYA
- Language
- EN