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Small-conductance Ca2+-activated K+ channels in bovine chromaffin cells by Antonio R. Artalejo; Antonio G. García; Erwin Neher is a Medicine article available to read on EtoBox.

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Simultaneous whole-cell patch-clamp and fura-2 fluorescence [Ca2+]~ measurements were used to characterize Ca2+-activated K + currents in cultured bovine chromaffin cells. Extracellular application of histamine (10 gM) induced a rise of [Ca2+]~ concomitantly with an outward current at holding potentials positive to -80 mV. The activation of the current reflected an increase in conductance, which did not depend on membrane potential in the range -80 mV to -40 mV. Increasing the extracellular K + concentration to 20 mM at the holding potential of -78 mV was associated with inwardly directed currents during the [Ca2+]i elevations induced either by histamine (10 gM) or short voltageclamp depolarizations. The current reversal potential was close to the K + equilibrium potential, being a function of external K + concentration. Current fluctuation analysis suggested a unit conductance of 3-5 pS for the channel that underlies this K + current. The current could be blocked by apamin (1 ~tM). Whole-cell current-clamp recordings showed that histamine (10 txM) application caused a transient hyperpolarization, which evolved in parallel with the [Ca2+]~ changes. It is proposed that a small-condu

Who reads Small-conductance Ca2+-activated K+ channels in bovine chromaffin cells?

Author

Antonio R. Artalejo; Antonio G. García; Erwin Neher

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0031-6768)

Published

1993

Language

EN

Field

Medicine (Life Sciences)