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Soy isoflavone daidzein protects Neuro2a cells from NO stress via activation of AMPK-PGC1α pathway followed by mitochondrial enhancement by Shogo Ito; Hiroko Sasaki; Takahiro Gotow; Isao Suetake; Kaoru Nagai is a Medicine article available to read on EtoBox.

What is Soy isoflavone daidzein protects Neuro2a cells from NO stress via activation of AMPK-PGC1α pathway followed by mitochondrial enhancement about?

Background Soy isoflavone daidzein protects cells from oxidative stress. Oxidative stress causes several neurodegenerative diseases via damaging mitochondria and inducing cell death. Methods In this study, we analyzed protective mechanisms of daidzein on nitric oxide (NO) induced neuronal Neuro2a cell death, and quantitated mitochondrial amount, that was analyzed by cytometric analysis and western blots of mitochondrial molecules. Results Daidzein reduced cell death against NO stress and increased mitochondrial amount even in the presence of NO. Regarding the mechanism of mitochondrial increase, daidzein increased the expression of mitochondrial biogenesis regulator Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) and induced nuclear translocation of PGC1α. Following this, we analyzed if the reduction of PGC1α reduces the protective function of daidzein against oxidative stress. Knockdown of PGC1α abolished the cell-protective function. It strongly suggests that PGC1α activation followed by the mitochondrial increase is critical for the cell protection. Next, we focused on 5′-adenosine monophosphate-activated protein kinase (AMPK) for clarifying how daid

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Author

Shogo Ito; Hiroko Sasaki; Takahiro Gotow; Isao Suetake; Kaoru Nagai

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)