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Monitoring Granule Formation in Anaerobic Upflow Bioreactors Using Oligonucleotide Hybridization Probes by D. Zheng; L.T. Angenent; L. Raskin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Monitoring Granule Formation in Anaerobic Upflow Bioreactors Using Oligonucleotide Hybridization Probes about?
## Abstract The process of granule formation in upflow anaerobic sludge blanket (UASB) reactors was studied using oligonucleotide hybridization probes. Two laboratory‐scale UASB reactors were inoculated with sieved primary anaerobic digester sludge from a municipal wastewater treatment plant and operated similarly except that reactor G was fed glucose, while reactor GP was fed glucose and propionate. Size measurements of cell aggregates and quantification of different populations of methanogens with membrane hybridization targeting the small‐subunit ribosomal RNA demonstrated that the increase in aggregate size was associated with an increase in the abundance of __Methanosaeta concilii__ in both reactors. In addition, fluorescence in situ hybridization showed that the major cell components of small aggregates collected during the early stages of reactor startup were __M. concilii__ cells. These results indicate that __M. concilii__ filaments act as nuclei for granular development. The increase in aggregate size was greater in reactor GP than in reactor G during the early stages of startup, suggesting that the presence of propionate‐oxidizing syntrophic consortia assisted the format
Who reads Monitoring Granule Formation in Anaerobic Upflow Bioreactors Using Oligonucleotide Hybridization Probes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- D. Zheng; L.T. Angenent; L. Raskin
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)