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Can I read Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation on EtoBox?
Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation by Robert N. Helsley; Se-Hyung Park; Hemendra J. Vekaria; Patrick G. Sullivan; Lindsey R. Conroy; Ramon C. Sun; María del Mar Romero; Laura Herrero; Joanna Bons; Christina D. King; Jacob Rose; Jesse G. Meyer; Birgit Schilling; C. Ronald Kahn; Samir Softic is a Medicine article available to read on EtoBox.
What is Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation about?
Background & Aims The consumption of sugar and a high-fat diet (HFD) promotes the development of obesity and metabolic dysfunction. Despite their well-known synergy, the mechanisms by which sugar worsens the outcomes associated with a HFD are largely elusive. Methods Six-week-old, male, C57Bl/6 J mice were fed either chow or a HFD and were provided with regular, fructose- or glucose-sweetened water. Moreover, cultured AML12 hepatocytes were engineered to overexpress ketohexokinase-C (KHK–C) using a lentivirus vector, while CRISPR-Cas9 was used to knockdown CPT1α. The cell culture experiments were complemented with in vivo studies using mice with hepatic overexpression of KHK–C and in mice with liver-specific CPT1α knockout. We used comprehensive metabolomics, electron microscopy, mitochondrial substrate phenotyping, proteomics and acetylome analysis to investigate underlying mechanisms. Results Fructose supplementation in mice fed normal chow and fructose or glucose supplementation in mice fed a HFD increase KHK–C, an enzyme that catalyzes the first step of fructolysis. Elevated KHK–C is associated with an increase in lipogenic proteins, such as ACLY, without affecting their mRNA e
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- Author
- Robert N. Helsley; Se-Hyung Park; Hemendra J. Vekaria; Patrick G. Sullivan; Lindsey R. Conroy; Ramon C. Sun; María del Mar Romero; Laura Herrero; Joanna Bons; Christina D. King; Jacob Rose; Jesse G. Meyer; Birgit Schilling; C. Ronald Kahn; Samir Softic
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Medicine (Health Sciences)