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Can I read A Semi-automated Protein Assay for Cell Cultures on EtoBox?
A Semi-automated Protein Assay for Cell Cultures by Charles Shopsis; Gregory J. Mackay is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A Semi-automated Protein Assay for Cell Cultures about?
A semi-automated modification of the protein determination procedure of O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951, J. Biol. Chem. 193, 265-275) is described. The assay is well suited to the analysis of the protein of adherent cultured cells. The procedure is carried out in 96-well microtest plates on protein solutions of 50 microliter or less, and can detect less than 0.5 micrograms of protein (equivalent to about 10(3) cultured cells). Optical densities are read and printed by an automatic microplate reader capable of processing 96 samples in less than 2 min.
Who reads A Semi-automated Protein Assay for Cell Cultures?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Charles Shopsis; Gregory J. Mackay
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1984
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)