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Umbilical Arterial Blood Flow in the Mouse Embryo During Development and Following Acutely Increased Heart Rate by Megan J MacLennan; Bradley B Keller is a Medicine article available to read on EtoBox.

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In anesthetized, pregnant ICR mice, we measured embryonic umbilical arterial velocity at baseline and during bipolar atrial or ventricular pacing. Pregnant mice were anesthetized with pentobarbital (60 mg/kg intraperitoneal) and ventilation was mechanically supported via a tracheotomy. Embryos were exposed through a mid-line laparotomy and regional hysterotomy. We recorded umbilical velocity using a 1-mm diameter piezoelectric crystal and 20-MHz, pulsed Doppler velocimeter at embryo day (ED) 10.5 (n ‫؍‬ 8), 12.5 (n ‫؍‬ 10), 13.5 (n ‫؍‬ 27), 14.5 (n ‫؍‬ 12), and 16.5 (n ‫؍‬ 17). We then acutely altered embryonic heart rate in n ‫؍‬ 8 ED 13.5 mouse embryos by bipolar atrial and ventricular pacing. Embryonic heart rate in this experimental preparation increased from 123 ؎ 7 to 193 ؎ 11 beats/min from ED 10.5 to 16.5 (p < 0.05). Peak instantaneous average velocity increased from 21 ؎ 2 to 55 ؎ 6 mm/s from ED 10.5 to 16.6 (p < 0.05), as did stroke volume and blood flow (p < 0.05 for each). In contrast to human umbilical arterial velocity profiles, significant forward diastolic flow was not seen at these stages, suggesting higher placental resistance in mice versus humans at comparable d

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Author

Megan J MacLennan; Bradley B Keller

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-5629)

Published

1999

Language

EN

Field

Medicine (Health Sciences)