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Determination of Midazolam in Human Plasma by Solid-phase Microextraction and Gas Chromatography/mass Spectrometry by Giampietro Frison; Luciano Tedeschi; Sergio Maietti; Santo Davide Ferrara is a Chemistry article available to read on EtoBox.
What is Determination of Midazolam in Human Plasma by Solid-phase Microextraction and Gas Chromatography/mass Spectrometry about?
## Abstract A new method is described for the qualitative and quantitative analysis of midazolam, a short‐acting 1,4‐imidazole benzodiazepine, in human plasma. It involves a plasma deproteinization step, solid‐phase microextraction (SPME) of midazolam using an 85‐μm polyacrylate fiber, and its detection by gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring (SIM) mode, using pinazepam as internal standard. The assay is linear over a midazolam plasma range of 1.5–300 ng/mL, relative intra‐ and inter‐assay standard deviations at 5 ng/mL are below 7%, and the limit of detection is 1 ng/mL. The method is simple, fast and sufficiently sensitive to be applied in clinical and forensic toxicology as well as for purposes of therapeutic drug monitoring. Copyright © 2001 John Wiley & Sons, Ltd.
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It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Giampietro Frison; Luciano Tedeschi; Sergio Maietti; Santo Davide Ferrara
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2001
- Language
- EN
- Field
- Chemistry (Physical Sciences)