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Schwann cells genetically engineered to express PSA show enhanced migratory potential without impairment of their myelinating ability in vitro by Alexandros A Lavdas; Isabelle Franceschini; Monique Dubois-Dalcq; Rebecca Matsas is a Neuroscience article available to read on EtoBox.
What is Schwann cells genetically engineered to express PSA show enhanced migratory potential without impairment of their myelinating ability in vitro about?
Schwann cells, the myelin-forming cells of the PNS, are attractive candidates for remyelination therapy as they can remyelinate CNS axons. Yet their integration in CNS tissue appears hampered, at least in part, by their limited motility in the CNS environment. As the polysialylated (PSA) form of NCAM regulates migration of neural precursors in the CNS and is not expressed by developing Schwann cells, we investigated whether conferring sustained expression of PSA to Schwann cells derived from postnatal rats enhances their motility. Cells were transduced with a retrovirus encoding polysialyl-transferase STX, an enzyme that synthesizes PSA on NCAM. Migration of wild type and transduced cells expressing STX or the marker gene alkaline phosphatase was examined using a gap bridging assay in dissociated cells and by grafting cells in slice cultures of postnatal brain. Migration of PSA expressing cells was significantly increased in both models, as compared to control cells, and this effect was abolished by endoneuraminidase-N stripping of PSA. PSA-positive Schwann cells retained the ability to differentiate in vitro and expressed the Krox20 and P zero myelination markers. When grafted in
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- Author
- Alexandros A Lavdas; Isabelle Franceschini; Monique Dubois-Dalcq; Rebecca Matsas
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)
- Published
- 2006
- Language
- EN
- Field
- Neuroscience (Life Sciences)