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Quantitative detection of micrometastases in the lymph nodes of gastric cancer patients with real-time RT-PCR: A comparative study with immunohistochemistry by Keisuke Kubota; Hayao Nakanishi; Naoki Hiki; Nobuyuki Shimizu; Eiichi Tsuji; Hirokazu Yamaguchi; Ken-ichi Mafune; Tsuyoshi Tange; Masae Tatematsu; Michio Kaminishi is a Medicine article available to read on EtoBox.
What is Quantitative detection of micrometastases in the lymph nodes of gastric cancer patients with real-time RT-PCR: A comparative study with immunohistochemistry about?
## Abstract Histologic examination lacks the sensitivity to detect micrometastases in gastric cancer lymph nodes. In the present study, we applied a real‐time RT‐PCR approach to the quantitative detection of micrometastases in gastric cancer lymph nodes and compared diagnostic power with routine histology and immunohistochemistry. We studied 392 lymph nodes from 21 gastric cancer patients who underwent curative surgery. Real‐time quantitative RT‐PCR was performed on a LightCycler instrument using a hybridization probe for carcinoembryonic antigen (CEA) and cytokeratin‐20 (CK20) as marker genes. Immunohistochemistry with antibodies to wide‐keratin was also performed in the lymph nodes to compare the sensitivity and specificity. Median (average) values of CEA mRNA in lymph nodes in patients with histology^+^, immunohistochemistry^+^/histology^−^, immunohistochemistry^−^/histology^−^ and negative control results were 4,600 (16,000), 200 (400), 0 (9.8) and 0 (0.6), respectively. There were some false‐negative results with simple CEA and CK20 real‐time RT‐PCR due to the presence of low gene‐expressing gastric cancers as revealed by CEA and CK20 immunohistochemistry. CEA in combination w
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- Author
- Keisuke Kubota; Hayao Nakanishi; Naoki Hiki; Nobuyuki Shimizu; Eiichi Tsuji; Hirokazu Yamaguchi; Ken-ichi Mafune; Tsuyoshi Tange; Masae Tatematsu; Michio Kaminishi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)