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Dynamic Monitoring of Cytosolic Glucose in Single Astrocytes by Mateja Prebil; Nina Vardjan; Jørgen Jensen; Robert Zorec; Marko Kreft is a Neuroscience article available to read on EtoBox.

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## Abstract It is becoming increasingly clear that astrocytes are no longer playing a subservient role to neurons in the central nervous system (CNS), and that these cells are being considered as active communication integrators. They respond to neurotransmitters by the regulated release of gliotransmitters. The delay between neurotransmitter activation and the release of gliotransmitters from astrocytes is in the time‐domain of subseconds, much slower than the submillisecond synaptic delay. Astrocytes also control microcirculation and provide metabolic support for neurons. However, the dynamics of their energy metabolic response to neurotransmitter application is not known. We here used a FRET glucose nanosensor to dynamically measure the cytosolic glucose concentration in single astrocytes. We show that following the adrenaline or noradrenaline stimulation the availability of cytosolic glucose is increased promptly after stimulation with a time‐constant of 116.7 s and 115.9 s, respectively. A decline in cytosolic glucose concentration with a time‐constant of 50.7 s was observed during glutamate and 16.7 s during lactate addition to astrocytes, when these were bathed in the presen

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Author

Mateja Prebil; Nina Vardjan; Jørgen Jensen; Robert Zorec; Marko Kreft

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2011

Language

EN

Field

Neuroscience (Life Sciences)