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Nanobiocatalysis for Protein Digestion in Proteomic Analysis by Jungbae Kim; Byoung Chan Kim; Daniel Lopez‐Ferrer; Konstantinos Petritis; Richard D. Smith is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Nanobiocatalysis for Protein Digestion in Proteomic Analysis about?

## Abstract The process of protein digestion is a critical step for successful protein identification in bottom‐up proteomic analyses. To substitute the present practice of in‐solution protein digestion, which is long, tedious, and difficult to automate, many efforts have been dedicated for the development of a rapid, recyclable and automated digestion system. Recent advances of nanobiocatalytic approaches have improved the performance of protein digestion by using various nanomaterials such as nanoporous materials, magnetic nanoparticles, and polymer nanofibers. Especially, the unprecedented success of trypsin stabilization in the form of trypsin‐coated nanofibers, showing no activity decrease under repeated uses for 1 year and retaining good resistance to proteolysis, has demonstrated its great potential to be employed in the development of automated, high‐throughput, and on‐line digestion systems. This review discusses recent developments of nanobiocatalytic approaches for the improved performance of protein digestion in speed, detection sensitivity, recyclability, and trypsin stability. In addition, we also introduce approaches for protein digestion under unconventional energy

Who reads Nanobiocatalysis for Protein Digestion in Proteomic Analysis?

Author

Jungbae Kim; Byoung Chan Kim; Daniel Lopez‐Ferrer; Konstantinos Petritis; Richard D. Smith

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)