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An efficient solubilization buffer for plant proteins focused in immobilized pH gradients by Valérie Méchin; Luciano Consoli; Martine Le Guilloux; Catherine Damerval is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The solubilization of a large array of proteins before electrophoresis itself is a very critical point for proteomic analyses. We compared the efficiency of several different solubilization buffers. From this work, we defined a very efficient solubilization buffer, including two chaotropes, two reducing agents (R2), two detergents (D2), and two kinds of carrier ampholytes in combination. This so‐called R2D2 buffer (5 M urea, 2 M thiourea, 2% 3‐[(3‐cholamidopropyl) dimethyl‐ammonio]‐1‐propane‐sulfonate, 2% __N__‐decyl‐__N,N__‐dimethyl‐3‐ammonio‐1‐propane‐sulfonate, 20 mM dithiothreitol, 5 mM Tris(2‐carboxyethyl) phosphine, 0.5% carrier ampholytes 4–6.5, 0.25% carrier ampholytes 3‐10) proved to be very efficient for a large range of different samples and allowed us to obtain two‐dimensional gels of high resolution and quality.

Who reads An efficient solubilization buffer for plant proteins focused in immobilized pH gradients?

Author

Valérie Méchin; Luciano Consoli; Martine Le Guilloux; Catherine Damerval

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)