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Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum 1 1 Introduction 1 2 Methods 2 2.1 Extraction of RNA, cDNA amplification and cloning 2 2.2 Expression and extraction of the target protein 2 2.3 Protein separation and purification 2 2.4 DLS of the recombinant protein 2 2.5 Enzyme assays 2 2.6 Molecular simulations 2 3 Results 3 3.1 Identification of PcRS1 genes in Polygonum cuspidatum and properties 3 3.2 Expression and purification of PcRS1 3 3.3 DLS detect aggregation status and enzyme assay of PcRS1 3 3.4 Molecular simulations 4 4 Discussion 7 5 Conclusion 7 Author statement 7 Declaration of competing interest 7 Acknowledgments 7 References 7
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- Language
- EN
- Category
- nonfiction
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