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Optimization of culture variables for improving glucoamylase production by alginate-entrapped Thermomucor indicae-seudaticae using statistical methods by Pardeep Kumar; T. Satyanarayana is a Engineering article available to read on EtoBox.

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Alginate-entrapped sporangiospores of Thermomucor indicae-seudaticae were used for the production of glucoamylase. The critical variables that affected glucoamylase production were identified by Plackett-Burman design (sucrose, yeast-extract, K 2 HPO 4 and asparagine) and further optimized by using a four factor central composite design (CCD) of response surface methodology (RSM). Immobilized sporangiospores secreted 41% and 60% higher glucoamylase titers in shake flasks and airlift fermenter, respectively, when the variables were used at their optimum levels (sucrose 3.0%, yeast-extract 0.2%, K 2 HPO 4 0.1% and asparagine 0.35%). Glucoamylase production (26.3 U ml À1 ) in the optimized medium was in good agreement with the values predicted by the quadratic model (26.7 U ml À1 ), thereby confirming its validity. The enzyme production was sustainable in flasks of higher volume and also airlift fermenter, and attained a peak within 32 h in the fermenter as compared to that of 48 h in shake flasks.

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Author

Pardeep Kumar; T. Satyanarayana

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0960-8524)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)