About this Biochemistry, Genetics and Molecular Biology article

Phytochemical Characterization, Functional Nutrition, and Anti-Diabetic Potentials of Leptadenia hastata (pers) Decne Leaves: In Silico and In Vitro Studies by Ifeoma Felicia Chukwuma; Florence Nkechi Nworah; Victor Onukwube Apeh; Kingsley Ozioma Omeje; Ekene John Nweze; Chukwudi Daniel Asogwa; Timothy Prince Chidike Ezeorba is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

The geometrical increase in diabetes mellitus (DM) and the undesirable side effects of synthetic drugs have intensified efforts to search for an effective and safe anti-diabetic therapy. This study aimed to identify the antioxidant and anti-diabetic agents in the ethanol extract of __Leptadenia hastata__ (EELH). The phytochemicals, antioxidant vitamins, and minerals present in EELH were determined using standard procedures to achieve this aim. Gas chromatography coupled with mass spectroscopy and flame ionization detector (GC-MS/GC-FID) was employed to identify bioactive compounds. An e-pharmacophore model was generated from the extra precision, and energy-minimized docked position of standard inhibitor, acarbose onto human pancreatic amylase (HPA, PDB-6OCN). It was used to screen the GC-MS/GC-FID library of compounds. The top-scoring compounds were subjected to glide XP-docking and prime MM-GBSA calculation with the Schrodinger suite-v12.4. The Adsorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) prediction of the best-fit compounds was made using SwissADME and PROTOX-II webservers. Further validation of the docking results was performed with the in vitro analysis

Author

Ifeoma Felicia Chukwuma; Florence Nkechi Nworah; Victor Onukwube Apeh; Kingsley Ozioma Omeje; Ekene John Nweze; Chukwudi Daniel Asogwa; Timothy Prince Chidike Ezeorba

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)