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In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism by Mariia A. Beliaeva; Florian Leisinger; Florian P. Seebeck is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism about?

Ergothioneine is a histidine-derived sulfur metabolite that is biosynthesized by bacteria and fungi. Plants and animals absorb ergothioneine as a micronutrient from their environment or nutrition. Several different mechanisms of microbial ergothioneine production have been described in the past ten years. Much less is known about the genetic and structural basis for ergothioneine catabolism. In this report, we describe the in vitro reconstitution of a five-step pathway that degrades ergothioneine to L-glutamate, trimethylamine, hydrogen sulfide, carbon dioxide, and ammonia. The first two steps are catalyzed by the two enzymes ergothionase and thiourocanate hydratase. These enzymes are closely related to the first two enzymes in histidine catabolism. However, the crystal structure of thiourocanate hydratase from the firmicute Paenibacillus sp. reveals specific structural features that strictly differentiate the activity of this enzyme from that of urocanate hydratases. The final two steps are catalyzed by metal-dependent hydrolases that share most homology with the last two enzymes in uracil catabolism. The early and late part of this pathway are connected by an entirely new enzyme

Who reads In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism?

Author

Mariia A. Beliaeva; Florian Leisinger; Florian P. Seebeck

Publisher

American Chemical Society (ACS)

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)