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Correlation of cellular differentiation in human colorectal carcinoma and adenoma cell lines with metabolite profiles determined by ^1^H magnetic resonance spectroscopy by Wanda B. MaCkinnon; Lily Huschtscha; Kathy Dent; Rebecca Hancock; Christos Paraskeva; Carolyn E. Mountford is a Medicine article available to read on EtoBox.
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## Abstract The aim was to determine whether proton magnetic resonance spectroscopy (MRS) could grade human colorectal cells of differing malignant potential. A cell model of tumour development and progression comprising 2 non‐tumorigenic adenoma lines and 4 carcinoma lines of increasing tumorigenicity was chosen. A gradual reduction in cellular differentiation and an accumulation of genetic alterations from adenoma to carcinoma characterized the selected cell lines. One‐dimensional and 2‐dimensional MRS showed that reduced differentiation in the cell model correlated with an increase in the levels of lipid, metabolites, the glycosylation intermediate uridine diphospho‐__N__‐acetylglucosamine and cell‐surface fucosylation. Mutations involving the K‐__ras__, APC and DCC genes are present both in adenoma‐ and in carcinoma‐derived lines in this model, but the first evidence of an abnormality in the __p53__ gene was concomitant with the cells' ability to grow as a tumour in athymic nude mice. This genetic change coincided with the detection, by MRS, of UDP‐hexose (ribose moiety, 2D MRS cross peak between H2 at 4.38 ppm and HI at 5.99 ppm) and the appearance of an additional fucosyl res
Who reads Correlation of cellular differentiation in human colorectal carcinoma and adenoma cell lines with metabolite profiles determined by ^1^H magnetic resonance spectroscopy?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Wanda B. MaCkinnon; Lily Huschtscha; Kathy Dent; Rebecca Hancock; Christos Paraskeva; Carolyn E. Mountford
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1994
- Language
- EN
- Field
- Medicine (Health Sciences)