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Neutrophils Generated in Vitro from Hematopoietic Stem Cells Isolated from Apheresis Samples and Umbilical Cord Blood Form Neutrophil Extracellular Traps by Rutuja Kuhikar; Nikhat Khan; Satyajeet P. Khare; Amit Fulzele; Sameer Melinkeri; Vaijayanti Kale; Lalita Limaye is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Neutrophils Generated in Vitro from Hematopoietic Stem Cells Isolated from Apheresis Samples and Umbilical Cord Blood Form Neutrophil Extracellular Traps about?
Neutrophils release neutrophil extracellular traps (NET) comprising of decondensed chromatin that immobilizes and kills pathogens. In vitro generation of neutrophils on a large scale from hematopoietic stem cells (HSCs) may be a useful strategy for treating neutropenic patients in future, though it is not in clinical practice yet. Microbial infections lead to major cause of morbidity and mortality in these patients. Despite the importance of NET in preventing infection, efficacy of in vitro-generated neutrophils from HSCs to form NET is not tested. We show that functional neutrophils could be generated in vitro from HSCs/MNCs isolated from umbilical cord blood (UCB) and apheresis-derived peripheral blood (APBL). Neutrophils generated from UCB showed properties comparable to those isolated from peripheral blood. We also show that isolation of HSCs is not absolutely essential for in vitro neutrophil generation. Further, we show that neutrophils generated from HSCs express PADI4 enzyme and their NET-forming ability is comparable to peripheral blood neutrophils. Taken together, our data show that fully functional neutrophils can be generated in vitro from HSCs. NET-forming ability of i
Who reads Neutrophils Generated in Vitro from Hematopoietic Stem Cells Isolated from Apheresis Samples and Umbilical Cord Blood Form Neutrophil Extracellular Traps?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Rutuja Kuhikar; Nikhat Khan; Satyajeet P. Khare; Amit Fulzele; Sameer Melinkeri; Vaijayanti Kale; Lalita Limaye
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)