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Enhanced Matrix‐assisted Laser Desorption/ionization Time‐of‐flight Mass Spectrometric Analysis of Bacteriophage Major Capsid Proteins with \ \ β\ \ ‐Mercaptoethanol Pretreatment by Casey R. McAlpin; Christopher R. Cox; Stephanie A. Matyi; Kent J. Voorhees is a Chemistry article available to read on EtoBox.

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## Abstract Bacteriophage (phage) proteins have been analyzed previously with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). However, analysis of phage major capsid proteins (MCPs) has been limited by the ability to reproducibly generate ions from MCP monomers. While the acidic conditions of MALDI‐TOF MS sample preparation have been shown to aid in disassembly of some phage capsids, many require further treatment to successfully liberate MCP monomers. The findings presented here suggest that __β__‐mercaptoethanol reduction of the disulfide bonds linking phage MCPs prior to mass spectrometric analysis results in significantly increased MALDI‐TOF MS sensitivity and reproducibility of __Yersinia pestis__‐specific phage protein profiles. Copyright © 2009 John Wiley & Sons, Ltd.

Who reads Enhanced Matrix‐assisted Laser Desorption/ionization Time‐of‐flight Mass Spectrometric Analysis of Bacteriophage Major Capsid Proteins with \ \ β\ \ ‐Mercaptoethanol Pretreatment?

Author

Casey R. McAlpin; Christopher R. Cox; Stephanie A. Matyi; Kent J. Voorhees

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)